Nucleofection effects on human T cells as a non-viral transfection technique
by
Güler, Seminay, author.
Title
:
Nucleofection effects on human T cells as a non-viral transfection technique
Author
:
Güler, Seminay, author.
Personal Author
:
Güler, Seminay, author.
Physical Description
:
x, 42 leaves:+ 1 computer laser optical disc.
Abstract
:
In times of quickly developing ways of immunological approaches applying on cells, nucleic acid delivery becomes a fundamental process. The ways of delivering nucleic acids mainly affects the consequence of immunological response. The nucleofection technology which is a modified form of electroporation is reliable method for transfection of hard to transfect cells. In the current study, we compared naive CD4 T cells and Jurkat cells in terms of nucleofection efficiency and cell viability. Naive CD4 T cells and Jurkat cells were transfected with pmax GFP at different time points. Out of 5 different nucleofection programs, one for naive CD4 T cells was identified. Jurkat cells were also transfected with one nucleofection program. Flow cytometric analyses of nucleofected naive CD4 T cells and Jurkat cells indicated that transfection efficiency significantly increased at 48h post transfection. We also differentiated naive CD4 T cells into T helper 17 cell phenotype by using certain stimulants and we activated Jurkat cells by using PMA and Ionomycin. We found that GFP was efficiently taken up by both T helper 17 cells and activated Jurkat cells. Therefore, nucleofection significantly holds important place in transfecting of hard to transfect cells including primary human CD4 T cells.
Subject Term
:
Transfection.
T cells -- Immunology.
Added Author
:
Nalbant Aldanmaz, Ayten
Added Corporate Author
:
İzmir Institute of Technology. Molecular Biology and Genetics.
Added Uniform Title
:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.
İzmir Institute of Technology: Molecular Biology and Genetics (Master).
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Thesis | T001573 | QH448.4 .G97 2016 | Tez Koleksiyonu |
IYTE Library | Supplementary CD-ROM | ROM2738 | QH448.4 .G97 2016 EK.1 | Tez Koleksiyonu |