PART I - Fundamentals 1  Network Theory in Computational and Systems Biology: Concepts and Applications. Marcelo Ferreira da Costa Gomes, Bruna de Paula Fonseca e Fonseca, and Tatiana Martins Tilli -- 2  In Search of an Organizing Principle in Biology: Thermodynamic Functions and Cancer. Edward Rietman and Jack A. Tuszynski -- 3  Systems Immunology. Melissa Lever, Helder I Nakaya -- 4 Mathematics and Computation for Modelling Transcriptional Regulatory Networks. Marcelo Trindade dos Santos, Ana Paula Nascimento, Fabrício A. B. Silva -- 5 Bio-modeling using Petri nets: A computational approach. Rafael Viana de Carvalho and Fons J. Verbeek -- PART II - Applied Systems Biology 6 The challenge of translating system biology in precision medicine of cancer. Alessandra Conforde, Milena Magalhães, Tatiana Tilli, Fabrício Alves, Nicolas Carels -- 7 Computational modeling of multiresistant bacteria.  Fabricio A. B. Silva, Thiago Merigueti, Thiago Giannini, Rafaela Brum, Ana Paula Nascimento, Kele Belloze, Floriano Silva, Rodolpho Albano, Marcelo Trindade dos Santos, Maria Clicia Castro, Márcio Argollo and Ana Paula D’A. Carvalho-Assef -- 8 Analysis Methods for Shotgun Metagenomics. Stephen Woloszynek, Gail Rosen, Zhengqiao Zhao, Dhantha Gunarathna , Alexandru Cristian, Joshua Chang Mell, Joshua P. Earl, Jacob Price, Christopher Sales -- 9 ANOCVA: a non-parametric statistical test to compare clustering structures. Alexandre Galvão Patriota, Davi Augusto Caetano de Jesus, and André Fujita -- 10 A path from specific enzyme to metabolic pathway modeling to access target relevance in molecular inhibitor development. Larissa Catharina Costa, Nicolas Carels, Marcio Argollo de Menezes -- 11 Mathematical-Computational Modeling in the Behavior Study of Neuronal Circuits of Repetitive Discharges. Maria Clicia Stelling de Castro, Dilson Silva, Celia Martins Cortez -- Index.  .