Adipocytes are the major energy depots which primarily compose adipose tissue in the body. They are mainly responsible for energy balance and also play a crucial role as endocrine and paracrine cells. Hypertrophy (cell size increase) and hyperplasia (cell number increase) are two mechanisms for adipose tissue growing, relating to some diseases such as obesity, osteoporosis, diabetes and anorexia nervosa. In this context, to detect and identify adipocytes has become critical to determine increase in cell size and cell number, thereby, it facilitates to understand mechanisms and process of adipocyte differentiation and provide to develop therapeutic strategies for the treatment and prevention of obesity and obesity-related diseases. Traditional or advanced techniques used for adipocyte detection and examination are available with their accomplished applications. However, they have some restrictions on adipocyte detection, such as being complicated and expensive operations or causing cell defects. Magnetic levitation is a novel technique with the capability of label-free, density-based detection using the principle of movement of the cells to lower magnetic field region in a paramagnetic medium. In this study, we used magnetic levitation system for detection of adipogenic-differentiated cells in heterogeneous cell populations. Results showed that levitation platform could detect the changes in lipid content of mesenchymal stem cells during adipogenic differentiation. This microfluidic system has a promising future with modification to sort adipogenic cells.