Iron and manganese accumulation in drinking water reservoirs is a challenging issue and should be controlled in order to prevent their adverse effects on human health. Accumulation of these elements not only clogs pipeline systems but also causes stains on fixtures and laundry. In addition, high concentrations of iron and manganese may lead to various health problems when ingested. This study focuses on the release mechanism of iron and manganese from sediments to water column and investigates methods to prevent this release. Effects of lack of hypolimnetic aeration, acid-base condition and thermal stratification on iron and manganese concentrations at water column were investigated through laboratory experiments. Experiments showed that total iron (Fe) and ferrous iron (Fe2+) concentrations in the water column decreased gradually following aeration in hypoxia conditions. A similar behavior was also observed for manganese concentrations. However, the concentration of dissolved manganese (Mn) in alkaline water condition is observed to be less than the aerated water case. This is an indication that iron and manganese react differently under different acidity of water in consideration.