Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
by
Ahmad, Misbha Ud Din
Title
:
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
Author
:
Ahmad, Misbha Ud Din
Personal Author
:
Ahmad, Misbha Ud Din
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (386 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2247868-1001 | XX(2247868.1) | JoVe Video Online |