Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
by
 
Bascuas, Thais

Title
: 
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Author
: 
Bascuas, Thais

Personal Author
: 
Bascuas, Thais

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (586 seconds)

Series
: 
Biology

General Note
: 
Title from resource description page

Abstract
: 
Here, a protocol to isolate and transfect primary iris and retinal pigment epithelial cells from various mammals (mice, rat, rabbit, pig, and bovine) is presented. The method is ideally suited to study ocular gene therapy approaches in various set-ups for ex vivo analyses and in vivo studies transferable to humans.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biology

Electronic Access
: 
https://www.jove.com/t/62145


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2248032-1001XX(2248032.1)JoVe Video Online