Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
by
 
Goering, Jeremy P.

Title
: 
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Author
: 
Goering, Jeremy P.

Personal Author
: 
Goering, Jeremy P.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (433 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
We present a protocol for isolation and culture of primary mouse embryonic palatal mesenchymal cells for time-lapse imaging of two-dimensional (2D) growth and wound-repair assays. We also provide the methodology for analysis of the time-lapse imaging data to determine cell-stream formation and directional motility.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/62151


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2248064-1001XX(2248064.1)JoVe Video Online