Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
by
 
Carvalho, Felipe A.

Title
: 
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Author
: 
Carvalho, Felipe A.

Personal Author
: 
Carvalho, Felipe A.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (344 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
Here, we describe the utilization of Grafix (Gradient Fixation), a glycerol gradient centrifugation in the presence of a crosslinker, to identify interactions between splicing factors that bind transiently to the spliceosome complex.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/61994


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2248200-1001XX(2248200.1)JoVe Video Online