Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
by
Arnold, Meghan Lee
Title
:
Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
Author
:
Arnold, Meghan Lee
Personal Author
:
Arnold, Meghan Lee
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (546 seconds)
Series
:
Neuroscience
General Note
:
Title from resource description page
Abstract
:
This protocol describes approaches for detection and quantitation of large aggregate and/or organelle extrusions (~4 µm) produced by C. elegans cells in the form of membrane-bound exophers. We describe strains, growth conditions, scoring criteria, timing, and microscopy considerations needed to facilitate dissection of this debris expulsion mechanism.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Neuroscience
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2248290-1001 | XX(2248290.1) | JoVe Video Online |