Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
by
Bose, Bipasha
Title
:
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
Author
:
Bose, Bipasha
Personal Author
:
Bose, Bipasha
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (779 seconds)
Series
:
Developmental Biology
General Note
:
Title from resource description page
Abstract
:
This protocol demonstrates the simultaneous detection of reactive oxygen species (ROS), live cells, and dead cells in live primary cultures from mouse ocular surface cells. 2',7'-Dichlorofluoresceindiacetate, propidium iodide, and Hoechst staining are used to assess the ROS, dead cells, and live cells, respectively, followed by imaging and analysis.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Developmental Biology
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2248887-1001 | XX(2248887.1) | JoVe Video Online |