Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
by
 
Dao, Sophia

Title
: 
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Author
: 
Dao, Sophia

Personal Author
: 
Dao, Sophia

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (392 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/60123


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249397-1001XX(2249397.1)JoVe Video Online