Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
by
 
Cestari, Igor

Title
: 
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Author
: 
Cestari, Igor

Personal Author
: 
Cestari, Igor

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (487 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
This protocol focuses on the identification of proteins that bind to inositol phosphates or phosphoinositides. It uses affinity chromatography with biotinylated inositol phosphates or phosphoinositides that are immobilized via streptavidin to agarose or magnetic beads. Inositol phosphate or phosphoinositide binding proteins are identified by Western blotting or mass spectrometry.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/59865


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249552-1001XX(2249552.1)JoVe Video Online