Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
by
Chen, Jiahuan
Title
:
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
Author
:
Chen, Jiahuan
Personal Author
:
Chen, Jiahuan
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (606 seconds)
Series
:
Immunology and Infection
General Note
:
Title from resource description page
Abstract
:
We present a protocol to functionalize atomic force microscope (AFM) cantilevers with a single T cell and bead particle for immunological studies. Procedures to probe single-pair T cell-dendritic cell binding by AFM and to monitor the real-time cellular response of macrophages to a single solid particle by AFM with fluorescence imaging are shown.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Immunology and Infection
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2249612-1001 | XX(2249612.1) | JoVe Video Online |