Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
by
 
Axmann, Markus

Title
: 
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Author
: 
Axmann, Markus

Personal Author
: 
Axmann, Markus

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (673 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/59573


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249858-1001XX(2249858.1)JoVe Video Online