Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
by
 
Fernandez, Josiah

Title
: 
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Author
: 
Fernandez, Josiah

Personal Author
: 
Fernandez, Josiah

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (754 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
This protocol describes how to isolate skin keratinocytes from mouse models, to stain with metal-tagged antibodies, and to analyze stained cells by mass cytometry in order to profile the expression pattern of proteins of interest in the different cell cycle phases.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/59353


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249862-1001XX(2249862.1)JoVe Video Online