Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
by
 
Bauer, Ingo

Title
: 
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Author
: 
Bauer, Ingo

Personal Author
: 
Bauer, Ingo

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (547 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
Class 1 histone deacetylases (HDACs) like RpdA have gained importance as potential targets to treat fungal infections. Here we present a protocol for the specific enrichment of TAP-tagged RpdA combined with an HDAC activity assay that allows in vitro efficacy testing of histone deacetylase inhibitors.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/59527


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249891-1001XX(2249891.1)JoVe Video Online