Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
by
 
Bouillier, Camille

Title
: 
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Author
: 
Bouillier, Camille

Personal Author
: 
Bouillier, Camille

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (708 seconds)

Series
: 
Immunology and Infection

General Note
: 
Title from resource description page

Abstract
: 
We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Immunology and Infection

Electronic Access
: 
https://www.jove.com/t/59218


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2249998-1001XX(2249998.1)JoVe Video Online