Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
by
 
Davydova, Maria

Title
: 
Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Author
: 
Davydova, Maria

Personal Author
: 
Davydova, Maria

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (527 seconds)

Series
: 
Chemistry

General Note
: 
Title from resource description page

Abstract
: 
In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Chemistry

Electronic Access
: 
https://www.jove.com/t/59063


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250105-1001XX(2250105.1)JoVe Video Online