Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
by
 
Andreassen, Patrick Rosendahl

Title
: 
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Author
: 
Andreassen, Patrick Rosendahl

Personal Author
: 
Andreassen, Patrick Rosendahl

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (424 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
Site-directed mutagenesis is a technique used to introduce specific mutations in deoxyribonucleic acid (DNA). This protocol describes how to do site-directed mutagenesis with a 2-step and 3-step polymerase chain reaction (PCR) based approach, which is applicable to any DNA fragment of interest.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/58996


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250218-1001XX(2250218.1)JoVe Video Online