A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
by
 
Arends, Eline J.

Title
: 
A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Author
: 
Arends, Eline J.

Personal Author
: 
Arends, Eline J.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (599 seconds)

Series
: 
Immunology and Infection

General Note
: 
Title from resource description page

Abstract
: 
This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Immunology and Infection

Electronic Access
: 
https://www.jove.com/t/59150


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250245-1001XX(2250245.1)JoVe Video Online