Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine
by
 
Arvin, Matthew C.

Title
: 
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Author
: 
Arvin, Matthew C.

Personal Author
: 
Arvin, Matthew C.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (648 seconds)

Series
: 
Neuroscience

General Note
: 
Title from resource description page

Abstract
: 
This article presents a method for studying nicotinic acetylcholine receptors (nAChRs) in mouse brain slices by nicotine uncaging. When coupled with simultaneous patch clamp recording and 2-photon laser scanning microscopy, nicotine uncaging connects nicotinic receptor function with cellular morphology, providing a deeper understanding of cholinergic neurobiology.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Neuroscience

Electronic Access
: 
https://www.jove.com/t/58873


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250269-1001XX(2250269.1)JoVe Video Online