Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
by
Bozóki, Beáta
Title
:
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Author
:
Bozóki, Beáta
Personal Author
:
Bozóki, Beáta
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (1163 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Here, we present the detailed procedure of a recently developed protease assay platform utilizing N-terminal hexahistidine/maltose-binding protein and fluorescent protein-fused recombinant substrates attached to the surface of nickel-nitrilotriacetic acid magnetic agarose beads. A subsequent in-gel analysis of the assay samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is also presented.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2250316-1001 | XX(2250316.1) | JoVe Video Online |