Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
by
Cordero, Teresa
Title
:
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Author
:
Cordero, Teresa
Personal Author
:
Cordero, Teresa
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (638 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2250461-1001 | XX(2250461.1) | JoVe Video Online |