Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
by
Bouchnak, Imen
Title
:
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Author
:
Bouchnak, Imen
Personal Author
:
Bouchnak, Imen
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (628 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Here, we describe a method to purify intact chloroplasts from Arabidopsis leaves and their three main sub-compartments (envelope, stroma, and thylakoids), using a combination of differential centrifugations, continuous Percoll gradients, and discontinuous sucrose gradients. The method is valuable for subplastidial and subcellular localization of proteins by immunoblotting and proteomics.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number |
---|
IYTE Library | Online Video | 2250622-1001 | XX(2250622.1) |