Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
by
Bhattacharya, Chayan
Title
:
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Author
:
Bhattacharya, Chayan
Personal Author
:
Bhattacharya, Chayan
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (633 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Here, we present a protocol for an efficient single step purification of the active untagged human ten-eleven translocation-2 (TET2) 5-methylcytosine dioxygenase using ion-exchange chromatography and its assay using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2250647-1001 | XX(2250647.1) | JoVe Video Online |