Rapid Isolation of the Mitoribosome from HEK Cells
by
Aibara, Shintaro
Title
:
Rapid Isolation of the Mitoribosome from HEK Cells
Author
:
Aibara, Shintaro
Personal Author
:
Aibara, Shintaro
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (573 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2250692-1001 | XX(2250692.1) | JoVe Video Online |