A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
by
 
Chorba, John S.

Title
: 
A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

Author
: 
Chorba, John S.

Personal Author
: 
Chorba, John S.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (494 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
This protocol presents a method to evaluate the proteolytic activity of an intrinsically low-activity, single turnover protease in a cellular context. Specifically, this method is applied to evaluate the proteolytic activity of PCSK9, a key driver of lipid metabolism whose proteolytic activity is required for its ultimate hypercholesterolemic function.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/58265


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250838-1001XX(2250838.1)JoVe Video Online