A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
by
 
Cai, Xiongwei

Title
: 
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Author
: 
Cai, Xiongwei

Personal Author
: 
Cai, Xiongwei

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (525 seconds)

Series
: 
Biochemistry

General Note
: 
Title from resource description page

Abstract
: 
A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample buffer.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Biochemistry

Electronic Access
: 
https://www.jove.com/t/56855


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2250875-1001XX(2250875.1)JoVe Video Online