Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
by
Becke, Tanja D.
Title
:
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Author
:
Becke, Tanja D.
Personal Author
:
Becke, Tanja D.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (673 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2250883-1001 | XX(2250883.1) | JoVe Video Online |