Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
by
Hadland, Brandon K.
Title
:
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
Author
:
Hadland, Brandon K.
Personal Author
:
Hadland, Brandon K.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (572 seconds)
Series
:
Developmental Biology
General Note
:
Title from resource description page
Abstract
:
Here we present methodology for the clonal analysis of hematopoietic stem cell precursors during murine embryonic development. We combine index sorting of single cells from the embryonic aorta-gonad-mesonephros region with endothelial cell co-culture and transplantation to characterize the phenotypic properties and engraftment potential of single hematopoietic precursors.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Developmental Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2251324-1001 | XX(2251324.1) | JoVe Video Online |