Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
by
Baumann, Tobias
Title
:
Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
Author
:
Baumann, Tobias
Personal Author
:
Baumann, Tobias
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (711 seconds)
Series
:
Bioengineering
General Note
:
Title from resource description page
Abstract
:
Synthetic biology enables the engineering of proteins with unprecedented properties using the co-translational insertion of non-canonical amino acids. Here, we presented how a spectrally red-shifted variant of a GFP-type fluorophore with novel fluorescence spectroscopic properties, termed "gold" fluorescent protein (GdFP), is produced in E. coli via selective pressure incorporation (SPI).
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Bioengineering
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2251381-1001 | XX(2251381.1) | JoVe Video Online |