Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
by
Amin, Mohammed A.
Title
:
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Author
:
Amin, Mohammed A.
Personal Author
:
Amin, Mohammed A.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (513 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
We present a protocol for double thymidine synchronization of HeLa cells followed by analysis using high resolution confocal microscopy. This method is key to obtaining large number of cells that proceed synchronously from S phase to mitosis, enabling studies on mitotic roles of multifunctional proteins which also possess interphase functions.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2251930-1001 | XX(2251930.1) | JoVe Video Online |