Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
by
 
Dege, Carissa

Title
: 
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Author
: 
Dege, Carissa

Personal Author
: 
Dege, Carissa

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (877 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/55196


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2252044-1001XX(2252044.1)JoVe Video Online