Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
by
Bardot, Evan
Title
:
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Author
:
Bardot, Evan
Personal Author
:
Bardot, Evan
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (582 seconds)
Series
:
Developmental Biology
General Note
:
Title from resource description page
Abstract
:
Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Developmental Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2252104-1001 | XX(2252104.1) | JoVe Video Online |