Generation of Genetically Modified Mice through the Microinjection of Oocytes
by
 
Delerue, Fabien

Title
Generation of Genetically Modified Mice through the Microinjection of Oocytes

Author
Delerue, Fabien

Personal Author
Delerue, Fabien

Publication Information
Cambridge, MA MyJoVE Corp 2016

Physical Description
online resource (619 seconds)

Series
Developmental Biology

General Note
Title from resource description page

Abstract
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

Reading Level
For undergraduate, graduate, and professional students

Subject Term
Developmental Biology

Electronic Access
https://www.jove.com/t/55765


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2252492-1001XX(2252492.1)JoVe Video Online