Single-cell Microinjection for Cell Communication Analysis
by
Alberto, Anael Viana Pinto
Title
:
Single-cell Microinjection for Cell Communication Analysis
Author
:
Alberto, Anael Viana Pinto
Personal Author
:
Alberto, Anael Viana Pinto
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (599 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
We describe here how to perform a single-cell microinjection of Lucifer Yellow to visualize cellular communication via gap-junctions in living cells, and provide some useful tips. We expect that this paper will help everyone to evaluate the degree of cellular coupling due to functional gap junctions. Everything described here could be, in principle, adapted to other fluorescent dyes with molecular weight below 1,000 Daltons.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2252856-1001 | XX(2252856.1) | JoVe Video Online |