Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
by
Balasubramaniam, Muthukumar
Title
:
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Author
:
Balasubramaniam, Muthukumar
Personal Author
:
Balasubramaniam, Muthukumar
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (634 seconds)
Series
:
Immunology and Infection
General Note
:
Title from resource description page
Abstract
:
This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Immunology and Infection
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2252871-1001 | XX(2252871.1) | JoVe Video Online |