Laboratory Scale Production and Purification of a Therapeutic Antibody
by
Elgundi, Zehra
Title
:
Laboratory Scale Production and Purification of a Therapeutic Antibody
Author
:
Elgundi, Zehra
Personal Author
:
Elgundi, Zehra
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (594 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2252970-1001 | XX(2252970.1) | JoVe Video Online |