Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
by
Dahl, Jan-Ulrik
Title
:
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Author
:
Dahl, Jan-Ulrik
Personal Author
:
Dahl, Jan-Ulrik
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (512 seconds)
Series
:
Biochemistry
General Note
:
Title from resource description page
Abstract
:
This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biochemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2253303-1001 | XX(2253303.1) | JoVe Video Online |