Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
by
Basak, Sandip
Title
:
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Author
:
Basak, Sandip
Personal Author
:
Basak, Sandip
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (679 seconds)
Series
:
Chemistry
General Note
:
Title from resource description page
Abstract
:
This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Chemistry
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2253650-1001 | XX(2253650.1) | JoVe Video Online |