Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
by
Bauman, Tyler M.
Title
:
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Author
:
Bauman, Tyler M.
Personal Author
:
Bauman, Tyler M.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (520 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2253895-1001 | XX(2253895.1) | JoVe Video Online |