Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
by
 
Blair, John D.

Title
: 
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Author
: 
Blair, John D.

Personal Author
: 
Blair, John D.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (591 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/53583


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2254074-1001XX(2254074.1)JoVe Video Online