Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
by
 
Benck, Charles J.

Title
: 
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Author
: 
Benck, Charles J.

Personal Author
: 
Benck, Charles J.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (431 seconds)

Series
: 
Immunology and Infection

General Note
: 
Title from resource description page

Abstract
: 
This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Immunology and Infection

Electronic Access
: 
https://www.jove.com/t/53638


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2254096-1001XX(2254096.1)JoVe Video Online