Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
by
Finney, Lydia A.
Title
:
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Author
:
Finney, Lydia A.
Personal Author
:
Finney, Lydia A.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (474 seconds)
Series
:
Chemistry
General Note
:
Title from resource description page
Abstract
:
Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Chemistry
Electronic Access
:
| Library | Material Type | Item Barcode | Shelf Number | Status |
|---|
| IYTE Library | Online Video | 2254798-1001 | XX(2254798.1) | JoVe Video Online |