Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
by
 
Cerbini, Trevor

Title
: 
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Author
: 
Cerbini, Trevor

Personal Author
: 
Cerbini, Trevor

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (448 seconds)

Series
: 
Developmental Biology

General Note
: 
Title from resource description page

Abstract
: 
TALEN-mediated gene editing at the safe harbor AAVS1 locus enables high-efficiency transgene addition in human iPSCs. This protocol describes the procedures for preparing iPSCs for TALEN and donor vector delivery, transfecting iPSCs, and selecting and isolating iPSC clones to achieve targeted integration of a GFP gene to generate reporter lines.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Developmental Biology

Electronic Access
: 
https://www.jove.com/t/52504


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2254902-1001XX(2254902.1)JoVe Video Online