High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
by
Baker, Jessica M.
Title
:
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Author
:
Baker, Jessica M.
Personal Author
:
Baker, Jessica M.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (775 seconds)
Series
:
Biology
General Note
:
Title from resource description page
Abstract
:
We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Biology
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2255535-1001 | XX(2255535.1) | JoVe Video Online |