Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
by
 
Butterick, Tammy A.

Title
: 
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Author
: 
Butterick, Tammy A.

Personal Author
: 
Butterick, Tammy A.

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (507 seconds)

Series
: 
Neuroscience

General Note
: 
Title from resource description page

Abstract
: 
Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Neuroscience

Electronic Access
: 
https://www.jove.com/t/51305


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2255654-1001XX(2255654.1)JoVe Video Online