Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
by
 
Cheng, Ziming

Title
: 
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Author
: 
Cheng, Ziming

Personal Author
: 
Cheng, Ziming

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (666 seconds)

Series
: 
Immunology and Infection

General Note
: 
Title from resource description page

Abstract
: 
Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Immunology and Infection

Electronic Access
: 
https://www.jove.com/t/50752


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2255779-1001XX(2255779.1)JoVe Video Online