Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
by
Burdick, Monica M.
Title
:
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Author
:
Burdick, Monica M.
Personal Author
:
Burdick, Monica M.
Publication Information
:
Cambridge, MA MyJoVE Corp 2016
Physical Description
:
online resource (633 seconds)
Series
:
Bioengineering
General Note
:
Title from resource description page
Abstract
:
Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
Reading Level
:
For undergraduate, graduate, and professional students
Subject Term
:
Bioengineering
Electronic Access
:
Library | Material Type | Item Barcode | Shelf Number | Status |
---|
IYTE Library | Online Video | 2255887-1001 | XX(2255887.1) | JoVe Video Online |