A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
by
 
Caglič, Dejan

Title
: 
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Author
: 
Caglič, Dejan

Personal Author
: 
Caglič, Dejan

Publication Information
: 
Cambridge, MA MyJoVE Corp 2016

Physical Description
: 
online resource (630 seconds)

Series
: 
Chemistry

General Note
: 
Title from resource description page

Abstract
: 
The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

Reading Level
: 
For undergraduate, graduate, and professional students

Subject Term
: 
Chemistry

Electronic Access
: 
https://www.jove.com/t/50908


LibraryMaterial TypeItem BarcodeShelf NumberStatus
IYTE LibraryOnline Video2255912-1001XX(2255912.1)JoVe Video Online